Part:BBa_K619893:Design

mutated prfA-UTR thermosensor from Listeria monocytogenes

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 18
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 18
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 18
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 18
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Our "wild-type" thermosensor (BBa-19889) Shine-Delagarno has been optimized for expression in E. coli. Otherwise, the "wild-type" sequence is identical to the thermosensor sequence from Listeria monocytogenes. Sequencing of this mutant revealed a single T56C bp change.

Source

prfA-UTR from Listeria monocytogenes